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[白术内酯]关苍术HPLC指纹图谱研究及白术内酯Ⅰ和Ⅲ的含量分析

发布时间:2015-09-25 17:25 |  点击次数:

【作者】 刁继红; 杨绍群; 刘爽; 梁刚; 梁贺; 张善玉;

【Author】 DIAO Ji-hong(Dept.of Pharmacy,The Affiliated Hospital of Yanbian University,Jilin Yanji 133000,China) YANG Shao-qun,LIU Shuang,LIANG Gang,LIANG He,ZHANG Shan-yu(Key Laboratory for Natural Resource of Changbai Mountain &Functional Molecules,Ministry of Education,Jilin Yanji 133000,China)

【机构】 延边大学附属医院药剂科; 长白山生物资源与功能分子教育部重点实验室;

【摘要】 目的:建立关苍术的高效液相色谱(HPLC)指纹图谱,并测定关苍术中白术内酯Ⅰ和Ⅲ的含量。方法:指纹图谱和含量测定研究的色谱柱分别为KromasilC18(250mm×4.6mm,5μm)和ODSHypersilC18(250mm×4.6mm,5μm),检测波长分别为240nm和220nm,流动相分别为水-乙腈(梯度洗脱)和甲醇-水(60:40,V/V),流速为1mL.min-1,进样量为10μL。结果:关苍术指纹图谱中共标定了9个共有指纹峰,10批样品的相似度均>0.9。白术内酯Ⅰ和Ⅲ的进样量分别在50.1~417.5ng(r=0.9997)和49.5~412.5ng(r=0.9998)范围内与各自峰面积积分值呈良好线性关系,平均加样回收率分别为101.02%和97.12%,RSD分别为4.32%和4.01%(n均为9)。结论:所建指纹图谱具有较好的精密度、重复性和稳定性;所建含量测定方法简单、灵敏、结果可靠,均可用于关苍术的品质评价和质量控制。 更多还原


【Abstract】 OBJECTIVE:To establish HPLC fingerprint of Atractylodes japonica,and to determine the contents of atractylenolideⅠand atractylenolide Ⅲ in A.japonica.METHODS:Fingerprint study and content determination were performed on Kromasil C 18 column(250 mm×4.6 mm,5 μm)and ODS Hypersil C 18 column(250 mm×4.6 mm,5 μm);the detection wavelength was set at 240 nm and 220 nm;mobile phase consisted of water-acetonitrile(gradient elution)and methanol-water(60:40,V/V)at the flow rate of 1 mL.min-1.The injection volume was 10 μL.RESULTS:There were 9 common peaks in the fingerprints of A.japonica,and the similarity of 10 batches of samples was higher than 0.9.The linear ranges were 50.1~417.5 ng(r=0.999 7)for atractylenolideⅠand 49.5~412.5 ng(r=0.999 8)for atractylenolide Ⅲ.The average recoveries were 101.02% and 97.12%,and RSDs were 4.32% and 4.01%,respectively(n=9).CONCLUSION:Established fingerprint has good precision,reproducibility and stability;the method is simple,sensitive and reliable.It is suitable for quality evaluation and control of A.japonica.

【药理作用】白术内酯Ⅰ能促进胃肠道消化吸收功能,是白术键脾的有效成分之-.具有抗炎、抗肿瘤作用,该成分还具有调节胃肠道功能和促进营养物质吸收的功能。